ABSTRACT
Objective: To explore the rationalization process of the project implementation and acceptance of water processing machine equipment installation so as to establish standardized installation acceptance system. Methods:Through combined the practices of medical equipment management of medical equipment department of hospital and blood purification centre in recent years, the installation and acceptance of water processing machine equipment was divided into 7 parts included 1)technical support pre-installation, 2)multi-party coordination, 3)implementation, inspection and confirmation of infrastructure projects, 4)achievement, hoisting and carrying of equipment, 5)installation and implementation of water processing machine, 6)acceptance of equipment, and 7)delivery of equipment to clinical usage. Besides, the whole process of installation and implementation was normalized. Results: Through the construction of institutionalization and processize for water processing machine of hospital, the efficiency and quality of the installation and acceptance for the equipment of water processing machine of blood purification centre were enhanced and improved. Conclusion: The institutionalization and processize of installation of water processing equipment in blood purification centre has important significance for giving full play to the function of equipment of water processing machine and quality control of equipment. And it can guarantee the medical quality of hospital, and contribute to effectively enhance the curative level of blood purification centre of hospital.
ABSTRACT
An efficient system of genetic transformation and plant regeneration via somatic embryogenesis was established in crownvetch (Coronilla varia L.) by infecting the segments of cotyledons and hypocotyls of 15d-old seedlings with Agrobacterium rhizogenes strain 15834. Hairy roots were produced directly from the wounded surface of the explants or via calluses on hormone-free Murashige and Skoog (MS) medium after infection by A. rhizogenes. Transformed roots grew rapidly either on solid or liquid MS medium, and exhibited typical hairy root phenotypes. The highest transformation frequency (87.4%) was achieved by preculturing cotyledons for 2d and pre-treating the A. rhizogenes with suitable concentration of acetosyringone at logarithmic phase (OD600 = 0.8). The embryogenic calluses with 100% induction frequency were induced from hairy roots on MS medium containing 0.2mg/L 2,4-D, 0.5mg/L NAA and 0.5mg/L KT. Globular-, heart-, torpedo-, and cotyledon shaped somatic embryos were produced orderly and developed into plantlets when transferred the embryogenic calluses on MS medium supplemented with 0.5mg/L KT, 0.2mg/L IBA and 300mg/L proline. The transformed plants did not show differences in morphology except abundant lateral root branches compared to the non-transformed plants. However, the contents of 3-nitropropanic acid in hairy roots and leaves of one of 5 transformed clones were 57.68% and 58.17% in roots and leaves of untransformed plants, respectively. Opine paper electrophoresis revealed the integration and expression of TR-DNA. PCR analysis confirmed that the TL-DNA including 654 bp rol B sequence was inserted into the genome of transformed hairy roots and their regenerated plants.
Subject(s)
Fabaceae , Genetics , Physiology , Plant Roots , Genetics , Physiology , Plants, Genetically Modified , Genetics , Regeneration , Rhizobium , Genetics , Tissue Culture Techniques , Transformation, GeneticABSTRACT
An efficient protocol for plant regeneration from protoplasts of the methionine resistant variant of Astragalus melilotoides was established. The friable calli induced from internode segments of variant plants were used for protoplast preparation. The protoplasts were isolated through enzyme digestion. Calli were formed after sustained divisions of protoplasts. High frequency of shoot differentiation was obtained from the protocalli on differentiated medium. The effects of different media, culturing methods and plating densities on protoplast divisions and plant regeneration were studied. The results show that agarose-beads culture method, KM8p medium supplemented with 1.0 mg/L 2,4-D, 0.5mg/L 6BA, 0.3 mol/L mannitol, 2% (W/V) sucrose and 500 mg/L casein hydrolysate at a plating density of 3 x 10(5)/mL are the appropriate conditions for protoplast division of the methionine resistant cell line. The division frequency is over 38%. The protoplast-regenerated plants still preserve resistance to methionine and ethionine.This research builds up the foundation for the resistant cell line as a parent of somatic hybridization.